Comparison of slow and fast neocortical neuron migration using a new in vitro model.

ABSTRACT:

Background

Mutations, toxic insults and radiation exposure are known to slow or arrest the migration of cortical neurons, in most cases by unknown mechanisms. The movement of migrating neurons is saltatory, reflecting the intermittent movement of the nucleus (nucleokinesis) within the confines of the plasma membrane. Each nucleokinetic movement is analogous to a step. Thus, average migration speed could be reduced by lowering step frequency and/or step distance.

Results

To assess the kinetic features of cortical neuron migration we developed a cell culture system that supports fiber-guided migration. In this system, the majority of fiber-apposed cells were neurons, expressed age-appropriate cortical-layer specific markers and migrated during a 30 min imaging period. Comparis

SUBMITTER: Nichols AJ 

PROVIDER: S-EPMC2440755 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature