The reticulon and DP1/Yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum.

ABSTRACT: We recently identified a class of membrane proteins, the reticulons and DP1/Yop1p, which shape the tubular endoplasmic reticulum (ER) in yeast and mammalian cells. These proteins are highly enriched in the tubular portions of the ER and virtually excluded from other regions. To understand how they promote tubule formation, we characterized their behavior in cellular membranes and addressed how their localization in the ER is determined. Using fluorescence recovery after photobleaching, we found that yeast Rtn1p and Yop1p are less mobile in the membrane than normal ER proteins. Sucrose gradient centrifugation and cross-linking analyses show that they form oligomers. Mutants of yeast Rtn1p, which no longer localize exclusively to the tubular ER or are even totally inactive in inducing ER tub

SUBMITTER: Shibata Y 

PROVIDER: S-EPMC2441541 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature