Systems analysis of quantitative shRNA-library screens identifies regulators of cell adhesion.

ABSTRACT:

Background

High throughput screens with RNA interference technology enable loss-of-function analyses of gene activities in mammalian cells. While the construction of genome-scale shRNA libraries has been successful, results of large-scale screening of those libraries can be difficult to analyze because of the relatively high noise levels and the fact that not all shRNAs in a library are equally effective in silencing gene expression.

Results

We have screened a library consisting of 43,828 shRNAs directed against 8,500 human genes for functions that are necessary in cell detachment induced by a constitutively activated c-Abl tyrosine kinase. To deal with the issues of noise and uncertainty of knockdown efficiencies, we employed an analytical strategy that combines quantitativ

SUBMITTER: Huang X 

PROVIDER: S-EPMC2441606 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature