Unilateral ablation of pre-Botzinger complex disrupts breathing during sleep but not wakefulness.

ABSTRACT:

Rationale

In adult rats, bilateral ablation of pre-Bötzinger complex (preBötC) neurokinin 1-expressing (NK1R) neurons leads to a progressive and irreversible disruption in breathing pattern, initially during sleep, eventually resulting in an ataxic breathing pattern during wakefulness.

Objectives

Here we determine whether ablation of fewer preBötC NK1R neurons leads to a persistent pattern of disordered breathing during sleep but not during wakefulness.

Methods

Adult male Sprague-Dawley rats (n = 12) were instrumented to record diaphragmatic, abdominal, and neck EMG, and EEG. Fourteen days later, a second surgery was performed to stereotaxically microinject into the preBötC on one side the toxin saporin conjugated to substance P (SP-SAP), which selectively ablates NK1

SUBMITTER: McKay LC 

PROVIDER: S-EPMC2441928 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature