The highest-copy repeats are methylated in the small genome of the early divergent vascular plant Selaginella moellendorffii.

ABSTRACT:

Background

The lycophyte Selaginella moellendorffii is a vascular plant that diverged from the fern/seed plant lineage at least 400 million years ago. Although genomic information for S. moellendorffii is starting to be produced, little is known about basic aspects of its molecular biology. In order to provide the first glimpse to the epigenetic landscape of this early divergent vascular plant, we used the methylation filtration technique. Methylation filtration genomic libraries select unmethylated DNA clones due to the presence of the methylation-dependent restriction endonuclease McrBC in the bacterial host.

Results

We conducted a characterization of the DNA methylation patterns of the S. moellendorffii genome by sequencing a set of S. moellendorffii shotgun genomic clone

SUBMITTER: Chan AP 

PROVIDER: S-EPMC2442089 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature