Eukaryotic elongation factor 1A interacts with sphingosine kinase and directly enhances its catalytic activity.

ABSTRACT: Sphingosine 1-phosphate (S1P) has many important roles in mammalian cells, including contributing to the control of cell survival and proliferation. S1P is generated by sphingosine kinases (SKs), of which two mammalian isoforms have been identified (SK1 and SK2). To gain a better understanding of SK regulation, we have used a yeast two-hybrid screen to identify SK1-interacting proteins and established elongation factor 1A (eEF1A) as one such protein that associates with both SK1 and SK2. We show the direct interaction of eEF1A with the SKs in vitro, whereas the physiological relevance of this association was demonstrated by co-immunoprecipitation of the endogenous proteins from cell lysates. Although the canonical role of eEF1A resides in protein synthesis, it has also been implicated in o

SUBMITTER: Leclercq TM 

PROVIDER: S-EPMC2442288 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature