Alterations in the proteome of pulmonary alveolar type II cells in the rat after hepatic ischemia-reperfusion.

ABSTRACT:

Objective

Hepatic ischemia-reperfusion can be associated with acute lung injury. Alveolar epithelial type II cells (ATII) play an important role in maintaining lung homeostasis in acute lung injury.

Design

To study potentially new mechanisms of hepatic ischemia-reperfusion-induced lung injury, we examined how liver ischemia-reperfusion altered the proteome of ATII.

Setting

Laboratory investigation.

Subjects

Spontaneously breathing male Zucker rats.

Interventions

Rats were anesthetized with isoflurane. The vascular supply to the left and medial lobe of the liver was clamped for 75 mins and then reperfused. Sham-operated rats were used as controls. After 8 hrs, rats were killed.

Measurements and main results

Bronchoalveolar lavage and differential c

SUBMITTER: Hirsch J 

PROVIDER: S-EPMC2442403 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature