Whole genome assessment of the retinal response to diabetes reveals a progressive neurovascular inflammatory response.

ABSTRACT:

Background

Despite advances in the understanding of diabetic retinopathy, the nature and time course of molecular changes in the retina with diabetes are incompletely described. This study characterized the functional and molecular phenotype of the retina with increasing durations of diabetes.

Results

Using the streptozotocin-induced rat model of diabetes, levels of retinal permeability, caspase activity, and gene expression were examined after 1 and 3 months of diabetes. Gene expression changes were identified by whole genome microarray and confirmed by qPCR in the same set of animals as used in the microarray analyses and subsequently validated in independent sets of animals. Increased levels of vascular permeability and caspase-3 activity were observed at 3 months of diab

SUBMITTER: Brucklacher RM 

PROVIDER: S-EPMC2442612 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature