Isolation, purification, and full NMR assignments of cyclopamine from Veratrum californicum.

ABSTRACT:

Background

The Hedgehog signaling pathway is essential for embryogenesis and for tissue homeostasis in the adult. However, it may induce malignancies in a number of tissues when constitutively activated, and it may also have a role in other forms of normal and maladaptive growth. Cyclopamine, a naturally occurring steroidal alkaloid, specifically inhibits the Hedgehog pathway by binding directly to Smoothened, an important Hedgehog response element. To use cyclopamine as a tool to explore and/or inhibit the Hedgehog pathway in vivo, a substantial quantity is required, and as a practical matter cyclopamine has been effectively unavailable for usage in animals larger than mice.

Results

In this paper, we report a rapid and efficient isolation and purification of large quantitie

SUBMITTER: Oatis JE 

PROVIDER: S-EPMC2442831 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature