Visualizing single molecules interacting with nuclear pore complexes by narrow-field epifluorescence microscopy.

ABSTRACT: The utility of single molecule fluorescence (SMF) for understanding biological reactions has been amply demonstrated by a diverse series of studies over the last decade. In large part, the molecules of interest have been limited to those within a small focal volume or near a surface to achieve the high sensitivity required for detecting the inherently weak signals arising from individual molecules. Consequently, the investigation of molecular behavior with high time and spatial resolution deep within cells using SMF has remained challenging. Recently, we demonstrated that narrow-field epifluorescence microscopy allows visualization of nucleocytoplasmic transport at the single cargo level. We describe here the methodological approach that yields 2 ms and approximately 15 nm resolution for a

SUBMITTER: Yang W 

PROVIDER: S-EPMC2442885 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature