Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution.

ABSTRACT:

Background

Vaccination of macaques with live attenuated simian immunodeficiency virus (SIV) provides significant protection against the wild-type virus. The use of a live attenuated human immunodeficiency virus (HIV) as AIDS vaccine in humans is however considered unsafe because of the risk that the attenuated virus may accumulate genetic changes during persistence and evolve to a pathogenic variant. We earlier presented a conditionally live HIV-1 variant that replicates exclusively in the presence of doxycycline (dox). Replication of this vaccine strain can be limited to the time that is needed to provide full protection through transient dox administration. Since the effectiveness and safety of such a conditionally live virus vaccine should be tested in macaques, we constructed a

SUBMITTER: Das AT 

PROVIDER: S-EPMC2443169 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature