A short-time scale colloidal system reveals early bacterial adhesion dynamics.

ABSTRACT: The development of bacteria on abiotic surfaces has important public health and sanitary consequences. However, despite several decades of study of bacterial adhesion to inert surfaces, the biophysical mechanisms governing this process remain poorly understood, due, in particular, to the lack of methodologies covering the appropriate time scale. Using micrometric colloidal surface particles and flow cytometry analysis, we developed a rapid multiparametric approach to studying early events in adhesion of the bacterium Escherichia coli. This approach simultaneously describes the kinetics and amplitude of early steps in adhesion, changes in physicochemical surface properties within the first few seconds of adhesion, and the self-association state of attached and free-floating cells. Examinati

SUBMITTER: Beloin C 

PROVIDER: S-EPMC2443189 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature