Mutations in the C-terminus of the X protein of hepatitis B virus regulate Wnt-5a expression in hepatoma Huh7 cells: cDNA microarray and proteomic analyses.

ABSTRACT:

Background

The hepatitis B virus x gene (HBx) is a promiscuous transactivator implicated in the development of hepatocellular carcinoma (HCC). The present study was designed to investigate the molecular events regulated by HBx.

Methods

Genomic and proteomic expression profiling was performed in Huh7 HCC cells transfected with HBx mutants with a C-terminal deletion. The gene and protein expression of wingless-type murine-mammary-tumour virus (MMTV) integration site family, member 5A (Wnt-5a) was validated by analyses of reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, western blot and immunohistochemistry.

Results

Differentially expressed genes and proteins were found in the transfected Huh7 HCC cells; most of them were involved in transcript

SUBMITTER: Liu X 

PROVIDER: S-EPMC2443277 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature