Polymorphism patterns in Duffy-binding protein among Thai Plasmodium vivax isolates.

ABSTRACT:

Background

The Duffy-binding protein II of Plasmodium vivax (PvDBPII) has been considered as an attractive target for vaccine-mediated immunity despite a possible highly polymorphic nature. Among seven PvDBP domains, domain II has been shown to exhibit a high rate of nonsynonymous polymorphism, which has been suggested to be a potential immune (antibody binding) evasion mechanism. This study aimed to determine the extent of genetic polymorphisms and positive natural selection at domain II of the PvDBP gene among a sampling of Thai P. vivax isolates.

Methods

The PvDBPII gene was PCR amplified and the patterns of polymorphisms were characterized from 30 Thai P. vivax isolates using DNA cloning and sequencing. Phylogenetic analysis of the sequences and positive selection were d

SUBMITTER: Gosi P 

PROVIDER: S-EPMC2443374 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature