Expression profiling of a high-fertility mouse line by microarray analysis and qPCR.

ABSTRACT:

Background

In a recent study it was demonstrated that a largely increased ovulation number is responsible for high prolificacy in two mouse lines selected for fertility performance. The objective of the present study was to identify genes that are involved in increasing the ovulation number in one of these lines, FL1. For differential expression profiling, ovaries of FL1 and of a non-selected control line, DUKsi, both lines derived from the same genetic pool, were analyzed with microarray analysis and quantitative polymerase chain reaction (qPCR). Ovaries from 30 animals of each line were collected at the metestrous stage, combined to 6 pools each, and processed for microarray analysis.

Results

The actual number of ova shed in FL1 exceeded that of the DUKsi control line more

SUBMITTER: Vanselow J 

PROVIDER: S-EPMC2443385 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature