Patterns of crystallin distribution in porcine eye lenses.

ABSTRACT:

Purpose

To measure the protein distribution patterns in single young porcine lenses.

Methods

Twenty fresh porcine lenses from 5 to 6 months old animals were fractionated into 8-10 concentric fractions by controlled dissolution in phosphate buffer. Proportions of soluble and insoluble protein were determined by Bradford assay. Water-soluble proteins in all layers were separated into HMW, MMW, and LMW fractions by size-exclusion HPLC and constituents of each class further characterized by SDS gel electrophoresis, as were the water-insoluble proteins. Size-exclusion fractions were further separated by reverse-phase HPLC and the molecular masses of each peak determined by MALDI-TOF mass spectrometry.

Results

The major soluble proteins in the porcine lens are beta-crystall

SUBMITTER: Keenan J 

PROVIDER: S-EPMC2443749 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature