The trypanosome transcriptome is remodelled during differentiation but displays limited responsiveness within life stages.

ABSTRACT:

Background

Trypanosomatids utilise polycistronic transcription for production of the vast majority of protein-coding mRNAs, which operates in the absence of gene-specific promoters. Resolution of nascent transcripts by polyadenylation and trans-splicing, together with specific rates of mRNA turnover, serve to generate steady state transcript levels that can differ in abundance across several orders of magnitude and can be developmentally regulated. We used a targeted oligonucleotide microarray, representing the strongly developmentally-regulated T. brucei membrane trafficking system and approximately 10% of the Trypanosoma brucei genome, to investigate both between-stage, or differentiation-dependent, transcriptome changes and within-stage flexibility in response to various challen

SUBMITTER: Koumandou VL 

PROVIDER: S-EPMC2443814 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature