Massively parallel interrogation of aptamer sequence, structure and function.

ABSTRACT:

Background

Optimization of high affinity reagents is a significant bottleneck in medicine and the life sciences. The ability to synthetically create thousands of permutations of a lead high-affinity reagent and survey the properties of individual permutations in parallel could potentially relieve this bottleneck. Aptamers are single stranded oligonucleotides affinity reagents isolated by in vitro selection processes and as a class have been shown to bind a wide variety of target molecules.

Methodology/principal findings

High density DNA microarray technology was used to synthesize, in situ, arrays of approximately 3,900 aptamer sequence permutations in triplicate. These sequences were interrogated on-chip for their ability to bind the fluorescently-labeled cognate target, im

SUBMITTER: Fischer NO 

PROVIDER: S-EPMC2444025 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature