Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli.

ABSTRACT: Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most gram-negative bacteria, and its structure and biosynthetic pathway are well known. Nevertheless, the mechanisms of transport and assembly of this molecule at the cell surface are poorly understood. The inner membrane (IM) transport protein MsbA is responsible for flipping LPS across the IM. Additional components of the LPS transport machinery downstream of MsbA have been identified, including the OM protein complex LptD/LptE (formerly Imp/RlpB), the periplasmic LptA protein, the IM-associated cytoplasmic ATP binding cassette protein LptB, and LptC (formerly YrbK), an essential IM component of the LPS transport machinery characterized in this work. Here we show that depletion of any of the proteins mention

SUBMITTER: Sperandeo P 

PROVIDER: S-EPMC2446812 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature