Genetic differentiation of Escherichia coli O157:H7 clades associated with human disease by real-time PCR.

ABSTRACT: The rapid and accurate identification of Escherichia coli O157:H7 strains is central to reducing the impact of outbreaks. A real-time PCR-based approach to differentiating major outbreak lineages of O157 with novel single-nucleotide polymorphisms is described. The utility of this method is in detection of hypervirulent strains in cases of clinical disease.

SUBMITTER: Riordan JT 

PROVIDER: S-EPMC2446876 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature