Molecular characterization of porcine picobirnaviruses and development of a specific reverse transcription-PCR assay.

ABSTRACT: The molecular characterization of partial- length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (>85% identity) to human PBV belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and Argentina showed that PBV circulate at high frequencies in piglets.

SUBMITTER: Carruyo GM 

PROVIDER: S-EPMC2446882 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature