Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair.

ABSTRACT: Despite the wealth of information available on the biochemical functions and our recent findings of its roles in genome stability and cancer avoidance of the structure-specific flap endonuclease 1 (FEN1), its cellular compartmentalization and dynamics corresponding to its involvement in various DNA metabolic pathways are not yet elucidated. Several years ago, we demonstrated that FEN1 migrates into the nucleus in response to DNA damage and under certain cell cycle conditions. In the current paper, we found that FEN1 is superaccumulated in the nucleolus and plays a role in the resolution of stalled DNA replication forks formed at the sites of natural replication fork barriers. In response to UV irradiation and upon phosphorylation, FEN1 migrates to nuclear plasma to participate in the resol

SUBMITTER: Guo Z 

PROVIDER: S-EPMC2447149 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature