Post-activation-mediated changes in opioid receptors detected by N-terminal antibodies.

ABSTRACT: The majority of studies examining activity-induced conformational changes in G protein-coupled receptors have focused on transmembrane helices or intracellular regions. Relatively few studies have examined the involvement of the extracellular region in general and the N-terminal region in particular in this process. To begin to address this, we generated a series of antibodies to the N-terminal region of opioid receptors. Characterization of these antibodies revealed that they differentially recognize activated receptors. Recently, we generated monoclonal antibodies that recognize regions proximal to glycosylation sites in the receptor N terminus. Characterization of these antibodies revealed that agonist treatment leads to a decrease in epitope recognition by the antibody presumably becau

SUBMITTER: Gupta A 

PROVIDER: S-EPMC2447646 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature