Reelin induces a radial glial phenotype in human neural progenitor cells by activation of Notch-1.

ABSTRACT:

Background

Reelin and Notch-1 signaling pathways have been recently found to be necessary to induce the expression of brain lipid binding protein (BLBP) and to promote the process extension and the maturation of the neuronal progenitors, the radial glial cells. In this study, we report the cross talk between these two pathways.

Results

Both in vitro Reelin treatment and overexpression of Notch-1 intracellular domain (NICD) induced BLBP expression and a radial glial phenotype in an immortalized human neural progenitor (HNP) cell line, isolated from the cortex of 14 weeks old fetus. Reelin treatment increased the level of NICD, indicating that Reelin signaling directly activates Notch-1. In addition, reducing NICD release, by inhibiting gamma-secretase activity, inhibited the

SUBMITTER: Keilani S 

PROVIDER: S-EPMC2447831 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature