Intraplastidial trafficking of a phage-type RNA polymerase is mediated by a thylakoid RING-H2 protein.

ABSTRACT: The plastid genome of dicotyledonous plants is transcribed by three different RNA polymerases; an eubacterial-type enzyme, PEP; and two phage-type enzymes, RPOTp and RPOTmp. RPOTp plays an important role in chloroplast transcription, biogenesis, and mesophyll cell proliferation. RPOTmp fulfills a specific function in the transcription of the rrn operon in proplasts/amyloplasts during seed imbibition/germination and a more general function in chloroplasts during later developmental stages. In chloroplasts, RPOTmp is tightly associated with thylakoid membranes indicating that functional switching of RPOTmp is connected to thylakoid association. By using the yeast two-hybrid system, we have identified two proteins that interact with RPOTmp. The two proteins are very similar, both characterize

SUBMITTER: Azevedo J 

PROVIDER: S-EPMC2449375 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature