Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation.

ABSTRACT:

Background

To identify the biochemical changes induced by sleep deprivation at a proteomic level, we compared the hippocampal proteome of rats either after 4 hours of sleep or sleep deprivation obtained by gentle handling. Because sleep deprivation might induce some stress, we also analyzed proteomic changes in rat adrenals in the same conditions. After sleep deprivation, proteins from both tissues were extracted and subjected to 2D-DIGE analysis followed by protein identification through mass spectrometry and database search.

Results

In the hippocampus, 87 spots showed significant variation between sleep and sleep deprivation, with more proteins showing higher abundance in the latter case. Of these, 16 proteins were present in sufficient amount for a sequencing attempt and

SUBMITTER: Poirrier JE 

PROVIDER: S-EPMC2453108 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature