Purification and characterization of recombinant human renin for X-ray crystallization studies.

ABSTRACT:

Background

The renin-angiotensin-aldosterone system (RAS) cascade is a major target for the clinical management of hypertension. Although inhibitors of various components of this cascade have been developed successfully, development of renin inhibitors has proven to be problematic. The development of these inhibitors has been hindered by poor bioavailability and complex synthesis. However, despite the challenges of designing renin inhibitors, the enzyme remains a promising target for the development of novel treatments for hypertension. X-ray crystallographic data could greatly assist the design and development of these inhibitors. Here we describe the purification and characterization of recombinant human renin for x-ray crystallization studies.

Results

A cDNA encoding the

SUBMITTER: Wu Z 

PROVIDER: S-EPMC2453115 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature