Structural model of a complex between the heterotrimeric G protein, Gsalpha, and tubulin.

ABSTRACT: A number of studies have demonstrated interplay between the cytoskeleton and G protein signaling. Many of these studies have determined a specific interaction between tubulin, the building block of microtubules, and G proteins. The alpha subunits of some heterotrimeric G proteins, including Gsalpha, have been shown to interact strongly with tubulin. Binding of Galpha to tubulin results in increased dynamicity of microtubules due to activation of GTPase of tubulin. Tubulin also activates Gsalpha via a direct transfer of GTP between these molecules. Structural insight into the interaction between tubulin and Gsalpha was required, and was determined, in this report, through biochemical and molecular docking techniques. Solid phase peptide arrays suggested that a portion of the amino terminus,

SUBMITTER: Layden BT 

PROVIDER: S-EPMC2453506 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature