Insulin-like growth factor-1 improves survival in sepsis via enhanced hepatic bacterial clearance.

ABSTRACT:

Rationale

Both insulin-like growth factor (IGF)-1 and bacterial clearance by Kupffer cells are significantly reduced in severe sepsis. Kupffer cell apoptosis is triggered by tumor necrosis factor (TNF)-alpha and activation of the PI-3 kinase pathway prevents TNF-induced Kupffer cell death.

Objectives

We evaluated if the marked decline in IGF-1 is related to bacterial clearance in sepsis.

Methods

Sepsis was induced in C57BL/6 mice by intratracheal inoculation with Pseudomonas aeruginosa (strain PA103). Some mice received IGF-1 24 mg/kg either before infection or 12 hours after infection. In vitro studies were performed using the clonal Kupffer cell line KC13-2.

Measurements and main results

Sepsis resulted in decreased levels of IGF-1. In vitro studies with KC13

SUBMITTER: Ashare A 

PROVIDER: S-EPMC2453509 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature