Human adenovirus type 36 enhances glucose uptake in diabetic and nondiabetic human skeletal muscle cells independent of insulin signaling.

ABSTRACT:

Objective

Human adenovirus type 36 (Ad-36) increases adiposity but improves insulin sensitivity in experimentally infected animals. We determined the ability of Ad-36 to increase glucose uptake by human primary skeletal muscle (HSKM) cells.

Research design and methods

The effect of Ad-36 on glucose uptake and cell signaling was determined in HSKM cells obtained from type 2 diabetic and healthy lean subjects. Ad-2, another human adenovirus, was used as a negative control. Gene expression and proteins of GLUT1 and GLUT4 were measured by real-time PCR and Western blotting. Role of insulin and Ras signaling pathways was determined in Ad-36-infected HSKM cells.

Results

Ad-36 and Ad-2 infections were confirmed by the presence of respective viral mRNA and protein expressions

SUBMITTER: Wang ZQ 

PROVIDER: S-EPMC2453622 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature