Alterations in mucosal immunity identified in the colon of patients with irritable bowel syndrome.

ABSTRACT:

Background & aims

Irritable bowel syndrome (IBS) has been associated with mucosal dysfunction, mild inflammation, and altered colonic bacteria. We used microarray expression profiling of sigmoid colon mucosa to assess whether there are stably expressed sets of genes that suggest there are objective molecular biomarkers associated with IBS.

Methods

Gene expression profiling was performed using Human Genome U133 Plus 2.0 (Affymetrix) GeneChips with RNA from sigmoid colon mucosal biopsy specimens from 36 IBS patients and 25 healthy control subjects. Real-time quantitative polymerase chain reaction was used to confirm the data in 12 genes of interest. Statistical methods for microarray data were applied to search for differentially expressed genes, and to assess the stability of

SUBMITTER: Aerssens J 

PROVIDER: S-EPMC2453689 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature