Proteomic analysis of lamellar bodies isolated from rat lungs.

ABSTRACT:

Background

Lamellar bodies are lysosome-related secretory granules and store lung surfactant in alveolar type II cells. To better understand the mechanisms of surfactant secretion, we carried out proteomic analyses of lamellar bodies isolated from rat lungs.

Results

With peptide mass fingerprinting by Matrix Assisted Laser Desorption/Ionization - Time of Flight mass spectrometry, 44 proteins were identified with high confidence. These proteins fell into diverse functional categories: surfactant-related, membrane trafficking, calcium binding, signal transduction, cell structure, ion channels, protein processing and miscellaneous. Selected proteins were verified by Western blot and immunohistochemistry.

Conclusion

This proteomic profiling of lamellar bodies provides a b

SUBMITTER: Wang P 

PROVIDER: S-EPMC2459160 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature