Proteomic analysis of Haloferax volcanii reveals salinity-mediated regulation of the stress response protein PspA.

ABSTRACT: A proteomic survey of the halophilic archaeon Haloferax volcanii was performed by comparative two-dimensional gel electrophoresis in order to determine the molecular effects of salt stress on the organism. Cells were grown under optimal (2.1 M) and high (3.5 M) NaCl conditions. From this analysis, over 44 protein spots responsive to these conditions were detected. These spots were excised, digested in-gel with trypsin, subjected to QSTAR tandem mass spectrometry (LC/MS/MS) analysis, and identified by comparing the MS/MS-derived peptide sequence to that deduced from the H. volcanii genome. Approximately 40 % of the proteins detected (18 in total) displayed differential abundance based on the detection of at least two peptide fragments per protein and overall MOWSE scores of >or=75 per prote

SUBMITTER: Bidle KA 

PROVIDER: S-EPMC2459321 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature