Performance comparison of two microarray platforms to assess differential gene expression in human monocyte and macrophage cells.

ABSTRACT:

Background

In this study we assessed the respective ability of Affymetrix and Illumina microarray methodologies to answer a relevant biological question, namely the change in gene expression between resting monocytes and macrophages derived from these monocytes. Five RNA samples for each type of cell were hybridized to the two platforms in parallel. In addition, a reference list of differentially expressed genes (DEG) was generated from a larger number of hybridizations (mRNA from 86 individuals) using the RNG/MRC two-color platform.

Results

Our results show an important overlap of the Illumina and Affymetrix DEG lists. In addition, more than 70% of the genes in these lists were also present in the reference list. Overall the two platforms had very similar performance in ter

SUBMITTER: Maouche S 

PROVIDER: S-EPMC2464609 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature