Cellular and molecular effects of nonreciprocal chromosome translocations in Saccharomyces cerevisiae.

ABSTRACT: Saccharomyces cerevisiae strains harboring a nonreciprocal, bridge-induced translocation (BIT) between chromosomes VIII and XV exhibited an abnormal phenotype comprising elongated buds and multibudded, unevenly nucleated pseudohyphae. In these cells, we found evidence of molecular effects elicited by the translocation event and specific for its particular genomic location. Expression of genes flanking both translocation breakpoints increased up to five times, correlating with an increased RNA polymerase II binding to their promoters and with their histone acetylation pattern. Microarray data, CHEF, and quantitative PCR confirmed the data on the dosage of genes present on the chromosomal regions involved in the translocation, indicating that telomeric fragments were either duplicated or int

SUBMITTER: Nikitin D 

PROVIDER: S-EPMC2474487 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature