Transcriptome analysis for Caenorhabditis elegans based on novel expressed sequence tags.

ABSTRACT:

Background

We have applied a high-throughput pyrosequencing technology for transcriptome profiling of Caenorhabditis elegans in its first larval stage. Using this approach, we have generated a large amount of data for expressed sequence tags, which provides an opportunity for the discovery of putative novel transcripts and alternative splice variants that could be developmentally specific to the first larval stage. This work also demonstrates the successful and efficient application of a next generation sequencing methodology.

Results

We have generated over 30 million bases of novel expressed sequence tags from first larval stage worms utilizing high-throughput sequencing technology. We have shown that approximately 14% of the newly sequenced expressed sequence tags map comp

SUBMITTER: Shin H 

PROVIDER: S-EPMC2474577 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature