A blackberry (Rubus L.) expressed sequence tag library for the development of simple sequence repeat markers.

ABSTRACT:

Background

The recent development of novel repeat-fruiting types of blackberry (Rubus L.) cultivars, combined with a long history of morphological marker-assisted selection for thornlessness by blackberry breeders, has given rise to increased interest in using molecular markers to facilitate blackberry breeding. Yet no genetic maps, molecular markers, or even sequences exist specifically for cultivated blackberry. The purpose of this study is to begin development of these tools by generating and annotating the first blackberry expressed sequence tag (EST) library, designing primers from the ESTs to amplify regions containing simple sequence repeats (SSR), and testing the usefulness of a subset of the EST-SSRs with two blackberry cultivars.

Results

A cDNA library of 18,432 cl

SUBMITTER: Lewers KS 

PROVIDER: S-EPMC2474608 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature