An optimized chloroplast DNA extraction protocol for grasses (Poaceae) proves suitable for whole plastid genome sequencing and SNP detection.

ABSTRACT:

Background

Obtaining chloroplast genome sequences is important to increase the knowledge about the fundamental biology of plastids, to understand evolutionary and ecological processes in the evolution of plants, to develop biotechnological applications (e.g. plastid engineering) and to improve the efficiency of breeding schemes. Extraction of pure chloroplast DNA is required for efficient sequencing of chloroplast genomes. Unfortunately, most protocols for extracting chloroplast DNA were developed for eudicots and do not produce sufficiently pure yields for a shotgun sequencing approach of whole plastid genomes from the monocot grasses.

Methodology/principal findings

We have developed a simple and inexpensive method to obtain chloroplast DNA from grass species by modifying a

SUBMITTER: Diekmann K 

PROVIDER: S-EPMC2474675 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature