High-precision, whole-genome sequencing of laboratory strains facilitates genetic studies.

ABSTRACT: Whole-genome sequencing is a powerful technique for obtaining the reference sequence information of multiple organisms. Its use can be dramatically expanded to rapidly identify genomic variations, which can be linked with phenotypes to obtain biological insights. We explored these potential applications using the emerging next-generation sequencing platform Solexa Genome Analyzer, and the well-characterized model bacterium Bacillus subtilis. Combining sequencing with experimental verification, we first improved the accuracy of the published sequence of the B. subtilis reference strain 168, then obtained sequences of multiple related laboratory strains and different isolates of each strain. This provides a framework for comparing the divergence between different laboratory strains and betwe

SUBMITTER: Srivatsan A 

PROVIDER: S-EPMC2474695 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature