Efficient genetic method for establishing Drosophila cell lines unlocks the potential to create lines of specific genotypes.

ABSTRACT: Analysis of cells in culture has made substantial contributions to biological research. The versatility and scale of in vitro manipulation and new applications such as high-throughput gene silencing screens ensure the continued importance of cell-culture studies. In comparison to mammalian systems, Drosophila cell culture is underdeveloped, primarily because there is no general genetic method for deriving new cell lines. Here we found expression of the conserved oncogene Ras(V12) (a constitutively activated form of Ras) profoundly influences the development of primary cultures derived from embryos. The cultures become confluent in about three weeks and can be passaged with great success. The lines have undergone more than 90 population doublings and therefore constitute continuous cell lin

SUBMITTER: Simcox A 

PROVIDER: S-EPMC2474701 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature