Exploring proteins in Anopheles gambiae male and female antennae through MALDI mass spectrometry profiling.

ABSTRACT: MALDI profiling and imaging mass spectrometry (IMS) are novel techniques for direct analysis of peptides and small proteins in biological tissues. In this work we applied them to the study of Anopheles gambiae antennae, with the aim of analysing expression of soluble proteins involved in olfaction perireceptor events. MALDI spectra obtained by direct profiling on single antennae and by the analysis of extracts, showed similar profiles, although spectra obtained through profiling had a richer ion population and higher signal to noise ratio. Male and female antennae showed distinct protein profiles. MALDI imaging experiments were also performed and differences were observed in the localization of some proteins. Two proteins were identified through high resolution measurement and top-down MS/

SUBMITTER: Dani FR 

PROVIDER: S-EPMC2474704 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature