Genome-wide analysis and expression profiling of the small heat shock proteins in zebrafish.

ABSTRACT: Small Heat Shock Proteins (sHSPs) have important roles in preventing disease and promoting resistance to environmental stressors. Mutations in any one of a number of sHSPs, including HSP27 (HSPB1), HSP22 (HSPB8), alphaA-crystallin (HSPB4), or alphaB-crystallin (HSPB5) can result in neuronal degeneration, myopathy, and/or cataract in humans. Ten sHSPs are known in humans, and thirteen have been identified in teleost fish. Here we report the identification of thirteen zebrafish sHSPs. Using a combination of phylogenetic analysis and analysis of synteny, we have determined that ten are likely orthologs of human sHSPs. We have used quantitative RT-PCR to determine the relative expression levels of all thirteen sHSPs during development and in response to heat shock. Our findings indicate that m

SUBMITTER: Elicker KS 

PROVIDER: S-EPMC2474744 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature