TDAG51 is an ERK signaling target that opposes ERK-mediated HME16C mammary epithelial cell transformation.

ABSTRACT:

Introduction

Signaling downstream of Ras is mediated by three major pathways, Raf/ERK, phosphatidylinositol 3 kinase (PI3K), and Ral guanine nucleotide exchange factor (RalGEF). Ras signal transduction pathways play an important role in breast cancer progression, as evidenced by the frequent over-expression of the Ras-activating epidermal growth factor receptors EGFR and ErbB2. Here we investigated which signal transduction pathways downstream of Ras contribute to EGFR-dependent transformation of telomerase-immortalized mammary epithelial cells HME16C. Furthermore, we examined whether a highly transcriptionally regulated ERK pathway target, PHLDA1 (TDAG51), suggested to be a tumor suppressor in breast cancer and melanoma, might modulate the transformation process.

Methods

Ce

SUBMITTER: Oberst MD 

PROVIDER: S-EPMC2474852 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature