Informatics strategies for large-scale novel cross-linking analysis.

ABSTRACT: The detection of protein interactions in biological systems represents a significant challenge for today's technology. Chemical cross-linking provides the potential to impart new chemical bonds in a complex system that result in mass changes in a set of tryptic peptides detected by mass spectrometry. However, system complexity and cross-linking product heterogeneity have precluded widespread chemical cross-linking use for large-scale identification of protein-protein interactions. The development of mass spectrometry identifiable cross-linkers called protein interaction reporters (PIRs) has enabled on-cell chemical cross-linking experiments with product type differentiation. However, the complex datasets resultant from PIR experiments demand new informatics capabilities to allow interpreta

SUBMITTER: Anderson GA 

PROVIDER: S-EPMC2475505 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature