Promoter library designed for fine-tuned gene expression in Pichia pastoris.

ABSTRACT: Although frequently used as protein production host, there is only a limited set of promoters available to drive the expression of recombinant proteins in Pichia pastoris. Fine-tuning of gene expression is often needed to maximize product yield and quality. However, for efficient knowledge-based engineering, a better understanding of promoter function is indispensable. Consequently, we created a promoter library by deletion and duplication of putative transcription factor-binding sites within the AOX1 promoter (P(AOX1)) sequence. This first library initially spanned an activity range between approximately 6% and >160% of the wild-type promoter activity. After characterization of the promoter library employing a green fluorescent protein (GFP) variant, the new regulatory toolbox was success

SUBMITTER: Hartner FS 

PROVIDER: S-EPMC2475614 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature