Classification and identification of bacteria by mass spectrometry and computational analysis.

ABSTRACT:

Background

In general, the definite determination of bacterial species is a tedious process and requires extensive manual labour. Novel technologies for bacterial detection and analysis can therefore help microbiologists in minimising their efforts in developing a number of microbiological applications.

Methodology

We present a robust, standardized procedure for automated bacterial analysis that is based on the detection of patterns of protein masses by MALDI mass spectrometry. We particularly applied the approach for classifying and identifying strains in species of the genus Erwinia. Many species of this genus are associated with disastrous plant diseases such as fire blight. Using our experimental procedure, we created a general bacterial mass spectra database that curren

SUBMITTER: Sauer S 

PROVIDER: S-EPMC2475672 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature