A fluorescence-based technique to construct size distributions from single-object measurements: application to the extrusion of lipid vesicles.

ABSTRACT: We report a novel approach to quantitatively determine complete size distributions of surface-bound objects using fluorescence microscopy. We measure the integrated intensity of single particles and relate it to their size by taking into account the object geometry and the illumination profile of the microscope, here a confocal laser scanning microscope. Polydisperse (as well as monodisperse) size distributions containing objects both below and above the optical resolution of the microscope are recorded and analyzed. The data is collected online within minutes, which allows the user to correlate the size of an object with the response from any given fluorescence-based biochemical assay. We measured the mean diameter of extruded fluorescently labeled lipid vesicles using the proposed method

SUBMITTER: Kunding AH 

PROVIDER: S-EPMC2479610 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature