Characterization of a restriction modification system from the commensal Escherichia coli strain A0 34/86 (O83:K24:H31).

ABSTRACT:

Background

Type I restriction-modification (R-M) systems are the most complex restriction enzymes discovered to date. Recent years have witnessed a renaissance of interest in R-M enzymes Type I. The massive ongoing sequencing programmes leading to discovery of, so far, more than 1 000 putative enzymes in a broad range of microorganisms including pathogenic bacteria, revealed that these enzymes are widely represented in nature. The aim of this study was characterisation of a putative R-M system EcoA0ORF42P identified in the commensal Escherichia coli A0 34/86 (O83: K24: H31) strain, which is efficiently used at Czech paediatric clinics for prophylaxis and treatment of nosocomial infections and diarrhoea of preterm and newborn infants.

Results

We have characterised a restricti

SUBMITTER: Weiserova M 

PROVIDER: S-EPMC2481252 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature