Specific and sensitive detection of H. pylori in biological specimens by real-time RT-PCR and in situ hybridization.

ABSTRACT: PCR detection of H. pylori in biological specimens is rendered difficult by the extensive polymorphism of H. pylori genes and the suppressed expression of some genes in many strains. The goal of the present study was to (1) define a domain of the 16S rRNA sequence that is both highly conserved among H. pylori strains and also specific to the species, and (2) to develop and validate specific and sensitive molecular methods for the detection of H. pylori. We used a combination of in silico and molecular approaches to achieve sensitive and specific detection of H. pylori in biologic media. We sequenced two isolates from patients living in different continents and demonstrated that a 546-bp domain of the H. pylori 16S rRNA sequence was conserved in those strains and in published sequences. Wit

SUBMITTER: Liu H 

PROVIDER: S-EPMC2481290 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature