PMC42, a breast progenitor cancer cell line, has normal-like mRNA and microRNA transcriptomes.

ABSTRACT:

Introduction

The use of cultured cell lines as model systems for normal tissue is limited by the molecular alterations accompanying the immortalisation process, including changes in the mRNA and microRNA (miRNA) repertoire. Therefore, identification of cell lines with normal-like expression profiles is of paramount importance in studies of normal gene regulation.

Methods

The mRNA and miRNA expression profiles of several breast cell lines of cancerous or normal origin were measured using printed slide arrays, Luminex bead arrays, and real-time reverse transcription-polymerase chain reaction.

Results

We demonstrate that the mRNA expression profiles of two breast cell lines are similar to that of normal breast tissue: HB4a, immortalised normal breast epithelium, and PMC4

SUBMITTER: Git A 

PROVIDER: S-EPMC2481505 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature